Clark Hall D21B

The 3i (Intelligent Imaging Innovations) Marianas Spinning Disk Confocal fluorescence microscope is perfect for gently imaging live samples as well as samples with large optical fields of view. With a fully motorized precision stage and fast acquisition, the inverted microscope is equipped for automating data collection from multiple locations, assembling 3D data sets, and collecting time-lapse videos. The SlideBook software works seamlessly for powerful and intuitive image analysis and allows many options for data export.

Setup Includes

• Zeiss Axio Observer 7 Advanced Marianas™ Microscope
  • Inverted Microscope with Transmitted Light LED Illuminator
  • Motorized Nosepiece and X,Y Stage
  • Objectives: 10x (air) ; 100x (oil)
• Yokogawa CSU-X1 A1H Spinning Disk Confocal Scanning Unit
  • Up to 2,000 fps Scanning Speed
  • Bandpass Filters: 617/73; 445/45; 525/30
  • Evolve 512 Delta 20MHz Back Illuminated EMCCD Camera
• LaserStack
  • Laser Lines: 405 nm 100 mW; 488 nm 50 mW; 532 nm 50 mW

3i Marianas SDCM

Introduction to Spinning Disc Confocal Microscopy

Quick Guide to the 3i Confocal Microscope